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In addition to its central role of energy storage and release, white adipose tissue (WAT) performs complex endocrine and immune activities. WAT produces physiologically active secretory proteins, including cytokines and complement factors. Furthermore, treatment of mammalian adipocytes with cytokines and inflammatory stimulators induces immune genes, suppresses regulators of adipocyte differentiation and activates lipolysis. Previously we reported up-regulation of immune genes in the course of in vitro development of Atlantic salmon white adipocytes. If WAT is immunoactive tissue in fish, excessive deposition of fat resulting from lipid-rich diets may imply risk for health of farmed fish. In this paper we investigated how lipopolysaccharide (LPS) affects immune activity in the adipose tissue-derived stromo-vascular fraction (aSVF) of Atlantic salmon. Experiments were performed with confluent cultures of proliferating preadipocytes. Exposure to LPS induced expression of immune genes, including TNFalpha and TNF-dependent genes, chemokines and receptors, NFkappaB related genes, matrix metalloproteinases and genes involved in eicosanoid metabolism. LPS decreased expression of adipocyte markers and genes involved in lipid metabolism, however, in parallel, it accelerated a number of transcriptional events that take place during the adipogenic differentiation of aSVF.

Original publication

DOI

10.1016/j.fsi.2010.07.026

Type

Journal article

Journal

Fish Shellfish Immunol

Publication Date

11/2010

Volume

29

Pages

817 - 824

Keywords

Adipocytes, White, Animals, Cells, Cultured, Chemokines, DNA Primers, Eicosanoids, Gene Expression Regulation, Immunohistochemistry, Lipopolysaccharides, Matrix Metalloproteinases, NF-kappa B, Polymerase Chain Reaction, Proliferating Cell Nuclear Antigen, Protein Array Analysis, Receptors, Chemokine, Salmo salar, Superoxide Dismutase, Tumor Necrosis Factor-alpha