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Novy, McNeal and Nicolle (NNN) medium and Evans' modified Tobie's medium are two conventional media for the isolation of Leishmania parasites in in-vitro cultures. Both are biphasic, with a solid layer of blood agar, and are normally prepared in glass test-tubes. In Sri Lanka at least, a monophasic microcapillary culture, based solely on RPMI 1640 medium supplemented with foetal calf serum, has been found simpler, more economical and more sensitive, for the isolation of L. donovani from skin lesions, than the use of Evans' modified Tobie's medium.

Original publication

DOI

10.1179/136485905X51364

Type

Journal article

Journal

Ann Trop Med Parasitol

Publication Date

09/2005

Volume

99

Pages

571 - 575

Keywords

Animals, Culture Media, Humans, Leishmania donovani, Leishmaniasis, Cutaneous, Sri Lanka, Time Factors