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Isolation and propagation of Leishmania parasites using conventional culture methods are difficult, especially under field conditions. Transportation of live parasites requires the maintenance of low temperatures, which increases the cost considerably. The present study demonstrates that monophasic micro capillary culture is a simpler, more economical and better alternative to using conventional Evan's modified Tobie's medium to isolate and transport the Leishmania parasite over long distances without the use of temperature control methods.

Type

Journal article

Journal

Ceylon Med J

Publication Date

06/2009

Volume

54

Pages

46 - 47

Keywords

Animals, Cost-Benefit Analysis, Humans, Leishmania, Leishmaniasis, Parasitic Sensitivity Tests, Sri Lanka, Temperature, Transportation