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Immune activation is a feature of dengue hemorrhagic fever (DHF) and CD8+ T cell responses in particular have been suggested as having a role in the vasculopathy that characterizes this disease. By phenotyping CD8+ T cells (CD38+/HLA-DR+, CD38+/Ki-67+, or HLA-DR+/Ki-67+) in serial blood samples from children with dengue, we found no evidence of increased CD8+ T cell activation prior to the commencement of resolution of viremia or hemoconcentration. Investigations with MHC class I tetramers to detect NS3(133-142)-specific CD8+ T cells in two independent cohorts of children suggested the commencement of hemoconcentration and thrombocytopenia in DHF patients generally begins before the appearance of measurable frequencies of NS3(133-142)-specific CD8+ T cells. The temporal mismatch between the appearance of measurable surface activated or NS3(133-142)-specific CD8+ T cells suggests that these cells are sequestered at sites of infection, have phenotypes not detected by our approach, or that other mechanisms independent of CD8+ T cells are responsible for early triggering of capillary leakage in children with DHF.

Original publication

DOI

10.4049/jimmunol.0903262

Type

Journal article

Journal

J Immunol

Publication Date

15/06/2010

Volume

184

Pages

7281 - 7287

Keywords

Adolescent, CD8-Positive T-Lymphocytes, Capillary Permeability, Cell Separation, Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Female, Flow Cytometry, Histocompatibility Antigens Class I, Humans, Lymphocyte Activation, Male, RNA Helicases, Reverse Transcriptase Polymerase Chain Reaction, Serine Endopeptidases, Severe Dengue, Viral Nonstructural Proteins