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Knock-in models of tumor-specific chromosomal translocations can generate lethal mutations. To circumvent this, a new conditional gene fusion model has been developed (invertor mice) and exemplified with the Ews-ERG fusion oncogene. An ERG segment, flanked by loxP sites, was knocked in to an intron of the Ews gene but in an inverted transcription orientation and lineage-specific Ews-ERG fusion created by Cre-mediated inversion. This invertor method is a completely conditional approach, applicable to any gene fusion, to emulate effects of translocations found in human cancers.

Original publication

DOI

10.1038/nmeth727

Type

Journal article

Journal

Nat Methods

Publication Date

01/2005

Volume

2

Pages

27 - 30

Keywords

Alleles, Animals, Base Sequence, Cell Separation, Chromosomes, DNA, Complementary, Flow Cytometry, Genetic Techniques, Humans, Introns, Mice, Mice, Inbred C57BL, Models, Genetic, Molecular Sequence Data, Mutation, RNA, Recombination, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Transcription, Genetic, Translocation, Genetic