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Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly review the current approaches to super-resolution optical microscopy and present the implementation of STED microscopy for novel insights into live cell mechanisms, with a focus on neurobiology and plasma membrane dynamics.

Original publication

DOI

10.1111/jnc.12243

Type

Journal article

Journal

J Neurochem

Publication Date

07/2013

Volume

126

Pages

203 - 212

Keywords

STED nanoscopy, fluorescence imaging, membrane dynamics, Animals, Cell Membrane, Cells, Humans, Microscopy, Fluorescence, Nanotechnology, Neurobiology