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The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.

Original publication

DOI

10.1038/nchem.1546

Type

Journal article

Journal

Nat Chem

Publication Date

02/2013

Volume

5

Pages

132 - 139

Keywords

Cell Line, Escherichia coli, Fluorescent Dyes, Gene Expression Regulation, Humans, Microscopy, Confocal, Proteins, Spectroscopy, Near-Infrared, Stereoisomerism