Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25-250 times faster recordings.DOI:http://dx.doi.org/10.7554/eLife.00248.001.

Original publication

DOI

10.7554/eLife.00248

Type

Journal article

Journal

Elife

Publication Date

31/12/2012

Volume

1

Keywords

GFP, None, confocal microscopy, fluorescent probes, live-cell imaging, nanoscopy, superresolution, Animals, Cell Line, Epithelial Cells, Escherichia coli, Gene Expression, Genes, Reporter, Green Fluorescent Proteins, Kidney, Light, Macropodidae, Microscopy, Fluorescence, Recombinant Proteins, Time Factors