Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Antisense technology has great potential for the control of RNA expression, but there remain few successful applications of the technology. Expressed antisense RNA can effectively down-regulate expression of a gene over long periods, but cannot differentiate partly identical sequences, such as the mRNA of fusion genes or those with point mutants. We have designed a structured form of expressed antisense, which can discriminate between highly similar mRNA molecules. These 'masked' antisense RNAs have most of the antisense sequence sequestered within duplex elements, leaving a short single-stranded region to initiate binding to target RNA. After contacting the correct target, the structured RNA can unravel, releasing the masked antisense region to form a stable duplex with the mRNA. We demonstrate that suitable masked antisense RNA can discriminate between the two forms of BCR-ABL mRNA that result from the Philadelphia chromosomal translocations, as well as discriminating the normal BCR and ABL mRNA.

Original publication

DOI

10.1038/sj.embor.embor633

Type

Journal article

Journal

EMBO Rep

Publication Date

07/2000

Volume

1

Pages

59 - 64

Keywords

Autoradiography, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Fusion Proteins, bcr-abl, HeLa Cells, Humans, Nucleic Acid Conformation, Nucleic Acid Hybridization, Oligonucleotides, RNA, Antisense, RNA, Messenger, Transfection