Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

OBJECTIVES: Anti-citrullinated protein/peptide antibodies (ACPA), a family of antibodies with overlapping specificities, represent a specific marker of rheumatoid arthritis (RA). The aim of the present study is to investigate the prevalence and clinical significance of IgG, IgA and IgM ACPA by a newly described assay employing a viral citrullinated peptide (VCP). METHODS: IgG, IgA and IgM anti-VCP antibodies have been measured in sera from 146 patients affected by RA and 404 controls, including 204 chronic arthritides, 111 connective tissue disorders and 89 healthy subjects. The affinity of the different isotypes for VCP was analysed by liquid phase inhibition assays. RESULTS: Among RA patients, 40 were single positive for IgG anti-VCP, five for IgA and 11 for IgM. Ten patients were double positive for IgG and IgA, four for IgG and IgM, six for IgA and IgM. In 15 RA patients IgG, IgA and IgM anti-VCP antibodies were detected. No correlation could be found between the isotype and the clinical manifestations or duration of the disease. IgA anti-VCP were strongly associated with RA, whereas IgM anti-VCP were detected also in a low percentage of systemic lupus erythematosus, psoriatic arthritis and mixed cryoglobulinaemia (MC) patients. IgG anti-VCP displayed a higher affinity for the antigen than IgA or IgM. CONCLUSIONS: These data show that anti-VCP of IgG and IgA isotype discriminate RA from other chronic arthritides and disease controls and suggest an independent production of each isotype.

Original publication

DOI

10.1093/rheumatology/kem193

Type

Journal article

Journal

Rheumatology (Oxford)

Publication Date

10/2007

Volume

46

Pages

1579 - 1582

Keywords

Adolescent, Adult, Aged, Aged, 80 and over, Antibodies, Viral, Antigens, Viral, Arthritis, Arthritis, Psoriatic, Arthritis, Rheumatoid, Autoimmune Diseases, Biomarkers, Connective Tissue Diseases, Diagnosis, Differential, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M, Lupus Erythematosus, Systemic, Middle Aged, Peptides, Cyclic, Rheumatoid Factor