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Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.

Original publication

DOI

10.1038/nmeth.1624

Type

Journal article

Journal

Nat Methods

Publication Date

05/06/2011

Volume

8

Pages

571 - 573

Keywords

Animals, Cell Line, Cell Membrane, Light, Lipids, Macropodidae, Microscopy, Fluorescence, Time Factors