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We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.

Original publication

DOI

10.1038/nmeth.1257

Type

Journal article

Journal

Nat Methods

Publication Date

11/2008

Volume

5

Pages

943 - 945

Keywords

Cell Line, Cell Survival, Humans, Microscopy, Fluorescence, Spectrometry, Fluorescence