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We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.

Original publication

DOI

10.1529/biophysj.107.112201

Type

Journal article

Journal

Biophys J

Publication Date

01/11/2007

Volume

93

Pages

3285 - 3290

Keywords

Animals, Cell Line, Escherichia coli, Fluorescent Dyes, Macropodidae, Microscopy, Fluorescence, Nanotechnology