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In the absence of replication of wells, empirical criteria for enzyme-linked immunospot (ELISpot) positivity use fixed differences or ratios between spot forming units (SFU) counts between test and control. We propose an alternative approach which first identifies the optimally variance-stabilizing transformation of the SFU counts, based on the Bland-Altman plot of the test and control wells. The second step is to derive a positivity threshold from the difference in between-plate distribution functions of the transformed test and control SFU counts. This method is illustrated using 1309 assay results from a cohort study of influenza in Vietnam in which some, but not all, of the peptide pools have clear tendencies for SFU counts to be higher in test than control wells.

Original publication

DOI

10.1016/j.jim.2013.02.014

Type

Conference paper

Publication Date

28/06/2013

Volume

392

Pages

57 - 62

Keywords

Adolescent, Adult, Aged, Aged, 80 and over, Analysis of Variance, Child, Child, Preschool, Cohort Studies, Enzyme-Linked Immunospot Assay, Humans, Influenza, Human, Middle Aged, Peptides, Vietnam, Young Adult