Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Blood and endothelial cells arise in close association in developing embryos, possibly from a shared precursor, the hemangioblast, or as hemogenic endothelium. The transcription factor, Scl/Tal1 (stem cell leukemia protein), is essential for hematopoiesis but thought to be required only for remodeling of endothelium in mouse embryos. By contrast, it has been implicated in hemangioblast formation in embryoid bodies. To resolve the role of scl in endothelial development, we knocked down its synthesis in zebrafish embryos where early precursors and later phenotypes can be more easily monitored. With respect to blood, the zebrafish morphants phenocopied the mouse knockout and positioned scl in the genetic hierarchy. Importantly, endothelial development was also clearly disrupted. Dorsal aorta formation was substantially compromised and gene expression in the posterior cardinal vein was abnormal. We conclude that scl is especially critical for the development of arteries where adult hematopoietic stem cells emerge, implicating scl in the formation of hemogenic endothelium.

Original publication

DOI

10.1182/blood-2004-09-3547

Type

Journal article

Journal

Blood

Publication Date

01/05/2005

Volume

105

Pages

3502 - 3511

Keywords

Animals, Aorta, Basic Helix-Loop-Helix Transcription Factors, Blood, Blood Circulation, DNA-Binding Proteins, Embryo, Nonmammalian, Endothelium, Vascular, Gene Expression Regulation, Developmental, Hematopoietic Stem Cells, Mice, Neovascularization, Physiologic, Proto-Oncogene Proteins, T-Cell Acute Lymphocytic Leukemia Protein 1, Transcription Factors, Zebrafish, Zebrafish Proteins