Dr. rer. nat.
Head of WIMM Genome Engineering
Genome Engineering Services at WIMM
The MRC WIMM Genome Engineering Facility is a central core service to establish genetically modified model systems. We are available to all researchers in MRC WIMM units as well as to other departments of the University of Oxford. Our team can advise on potential experimental strategies, provide validated reagents and assemble custom repair and targeting donors. We generate custom cell lines by CRISPR/Cas9 gene editing and, in collaboration with the MRC WIMM Transgenic Facility, we generate custom mouse models using our Cas9-assisted mESC targeting pipeline. We also provide expertise in CRISPR screening and virus production in close collaboration with the MRC WIMM Virus and Screening Facility. Please feel free to contact us at any time to find out how we can help.
2003: Dr. rer. nat. from the Albert Ludwigs University of Freiburg, Germany. Functional studies of transcriptional co-repressors, characterisation of the novel INHAT repressor NIR.
2005 - 2008: Postdoctoral fellowship at the Friedrich Miescher Institute for Biomedical Research in Basel, Switzerland. Research on the function of histone methyltransferases in early mouse development.
2008 - 2014: Head of the Gene Expression Services at the EMBL Rome outstation. Responsible for the generation of classical and BAC transgenic constructs. Responsible for generation of custom targeting constructs (KO, cKO, KI, cKI). In those pre-CRISPR days, most of the work was done by advanced recombineering and classic HDR techniques.
2014 – today: Head of the MRC WIMM Genome Engineering Facility. Generation of cell and mouse model systems. The rapid development of the CRISPR/Cas9 platform made cell line engineering a fundamental new part of the work. Besides the assembly of plasmid constructs, the GE core facility offers a wide portfolio of services: KO, KI, cKO, cKI, COIN alleles, SNP modelling, Base Editing, Prime Editing, CRISPRi/a approaches and custom viral screening.
Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions.
Blayney J. et al, (2020), Methods Protoc, 3
RIG-I Plays a Dominant Role in the Induction of Transcriptional Changes in Zika Virus-Infected Cells, which Protect from Virus-Induced Cell Death.
Schilling M. et al, (2020), Cells, 9
An integrated platform to systematically identify causal variants and genes for polygenic human traits
Downes D. et al, (2019)
SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells.
Chagraoui H. et al, (2018), Nat Commun, 9
Genetic abrogation of immune checkpoints in antigen-specific cytotoxic T-lymphocyte as a potential alternative to blockade immunotherapy.
Zhang C. et al, (2018), Sci Rep, 8