Research groups
Colleges
Jacqueline Boultwood
BSc (Hons), PhD
Professor of Molecular Haematology
- Director of Graduate Studies in the Medical Sciences Division
Molecular pathogenesis of myeloid malignancies
My research studies concern the investigation of the molecular pathogenesis of myeloid malignancies, including the myelodysplastic syndromes (MDS) and acute myeloid leukaemia (AML).
My work has focused on MDS, a heterogeneous group of clonal myeloid disorders. My studies have been instrumental in the determination of the molecular pathogenesis of several subtypes of MDS including the 5q- syndrome and refractory anaemia with ring sideroblasts (RARS). Our research identified the commonly deleted region of the del(5q) in the 5q- syndrome and showed that p53 activation underlies the anaemia in this disorder.
Using next generation sequencing technology we have illuminated the molecular landscape of MDS and the genetic basis of disease progression to AML. Our study of the MDS transcriptome has yielded valuable insights into the molecular pathophysiology of MDS, and has identified new prognostic markers and therapeutic targets for this disorder. Our work has provided deep insights into how gene mutations drive the changes in the MDS transcriptome.
Splicing factor genes are the most commonly mutated genes in MDS, and we have identified novel aberrantly spliced genes and dysregulated pathways in splicing factor mutant MDS haematopoietic stem and progenitor cells. Mutation of the splicing factor SF3B1 is strongly associated with the MDS subtype RARS and we have implicated aberrant splicing of the downstream target gene ABCB7 in the pathophysiology of RARS. In a recent collaborative study we have shown that U2AF1 mutations induce oncogenic isoforms of IRAK4 in myeloid malignancies that are therapeutically targetable. We are currently using single-cell analysis to determine the transcriptomic changes occurring in the stem cell population of splicing factor mutant MDS. Our data have critical implications for understanding MDS phenotypic heterogeneity and support the development of therapies targeting splicing abnormalities.
We are also using CRISPR/Cas9 genome editing together with induced pluripotent stem cell (iPSC) technology to model chronic myelomonocytic leukaemia and for drug discovery.
I have served on several advisory committees and scientific panels, including for NHLBI, Leukaemia UK and the International Working Group for the Prognosis of MDS. I am a committee member and trustee of the UK MDS Forum. I have served as a member of Editorial Boards of many scientific journals, including the British Journal of Haematology, Haematologica and Blood, and as an Associate Editor of Molecular Biotechnology.
Key publications
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U2AF1 mutations induce oncogenic IRAK4 isoforms and activate innate immune pathways in myeloid malignancies.
Journal article
Smith MA. et al, (2019), Nat Cell Biol, 21, 640 - 650
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Impact of spliceosome mutations on RNA splicing in myelodysplasia: dysregulated genes/pathways and clinical associations.
Journal article
Pellagatti A. et al, (2018), Blood, 132, 1225 - 1240
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The U2AF1S34F mutation induces lineage-specific splicing alterations in myelodysplastic syndromes.
Journal article
Yip BH. et al, (2017), J Clin Invest, 127, 2206 - 2221
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Combining gene mutation with gene expression data improves outcome prediction in myelodysplastic syndromes.
Journal article
Gerstung M. et al, (2015), Nat Commun, 6
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Identification of gene expression-based prognostic markers in the hematopoietic stem cells of patients with myelodysplastic syndromes.
Journal article
Pellagatti A. et al, (2013), J Clin Oncol, 31, 3557 - 3564
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A p53-dependent mechanism underlies macrocytic anemia in a mouse model of human 5q- syndrome.
Journal article
Barlow JL. et al, (2010), Nat Med, 16, 59 - 66
Recent publications
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Author Correction: Implications of TP53 allelic state for genome stability, clinical presentation and outcomes in myelodysplastic syndromes.
Journal article
Bernard E. et al, (2021), Nat Med
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The utilisation of glutamine and glucose by a 3-D tumour model trapped in quiescence.
Journal article
Smith H. et al, (2021), Int J Biochem Cell Biol
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SF3B1 mutant myelodysplastic syndrome: Recent advances.
Journal article
Pellagatti A. and Boultwood J., (2020), Adv Biol Regul
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Sex differences in oncogenic mutational processes
Journal article
Li CH. et al, (2020), Nature Communications, 11
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Spliceosome mutations: 1 plus 1 does not always equal 2.
Journal article
Pellagatti A. and Boultwood J., (2020), Blood, 136, 1471 - 1472
ORCID
0000-0002-4330-2928