Dr Amlan Roychowdhury
PhD, MSc, BSc (Hons)
Understanding transcriptional regulation in hematopoietic development from a structural aspect
I am a structural biologist currently working as a Postdoctoral Scientist at the Porcher Lab (WIMM). I aim to understand the role of transcriptional regulation in the early stage of hematopoietic development, it deals with determining the structure of large DNA-protein complexes using Cryo-EM and X-ray crystallography. Previously I worked as a Postdoctoral Researcher in Ecole Polytechnique, France (Laboratoire de Biologie Structurale de la Cellule; Team: Dr. Marc Graille) for four years and worked on protein complexes, involved in ribosome biogenesis and maturation. I did my Ph.D. in Structural biology (protein X-ray crystallography) from the Indian Institute of Technology, Kharagpur.
I am also working with Dr. Denis Ptchelkine (Co-Supervisor) to maintain our Protein purification facility and insect cell culture facility (baculovirus expression system) in WIMM.
The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis.
Roychowdhury A. et al, (2019), Nucleic Acids Res, 47, 7548 - 7563
Elucidation of the mechanism of disulfide exchange between staphylococcal thioredoxin2 and thioredoxin reductase2: A structural insight.
Bose M. et al, (2019), Biochimie, 160, 1 - 13
Reconstitution of the RNA-dependent RNA polymerase activity of Antheraea mylitta cypovirus in vitro using separately expressed different functional domains of the enzyme.
Kundu A. et al, (2016), J Gen Virol, 97, 1709 - 1719
Complete catalytic cycle of cofactor-independent phosphoglycerate mutase involves a spring-loaded mechanism.
Roychowdhury A. et al, (2015), FEBS J, 282, 1097 - 1110
Expression, purification, crystallization and preliminary X-ray diffraction studies of phosphoglycerate mutase from Staphylococcus aureus NCTC8325.
Roychowdhury A. et al, (2014), Acta Crystallogr F Struct Biol Commun, 70, 53 - 56