- Vyas Group: Normal and Malignant Haematopoiesis Lab Research Group
BSc Hons, MSc
As a Research Assistant to the Vyas group, I offer support to a number of ongoing projects in the lab utilising a range of skills and techniques, including single cell and bulk whole genome amplification, single cell genotyping, PCR, Next Generation sequencing, Sanger sequencing, flow cytometry and bioinformatics.
My scientific focus has been investigating the clonality of AML – deciphering the evolution of clones from diagnosis through to remission and subsequent relapse. I am jointly responsible for genotyping AML samples via large scale targeted re-sequencing using the Fluidigm Access Array system and all down-stream bioinformatic analysis, using programmes such as cutadapt, fastqc, stampy, samtools, Integrative Genomics Viewer etc. My other responsibilities include the routine screening of GATA1 for mutations in the context of TAM (Transient Abnormal Myelopoiesis) and ML-DS (Myeloid Leukaemia in Down Syndrome). This is based on a clinical need in patients with Trisomy 21 with samples coming from across Europe. Liaison with clinical teams has allowed me to immerse myself in clinical nomenclature and see the benefits of a sensitive molecular test to accurately measure minimal residual disease. I am currently optimising a protocol for reduced representation bisulfite sequencing (RRBS) on low cell numbers to allow the lab to implement this technique using patient samples in order to analyse methylation status alongside our current genotyping capabilities.
I have an undergraduate degree in Combined Honours (BSc Hons) from the University of Liverpool studying Biological Sciences and Psychology. I completed a Masters Degree (MSc) at University College London studying the Genetics of Human Disease, before taking up my post at the University of Oxford with Professor Vyas in December 2014.