I joined the Milne lab in 2018 after completing my PhD in the lab of Joan Boyes at the University of Leeds in 2018, where I investigated the temporal regulation of V(D)J recombination. My current research investigates the emergence of novel enhancers in leukaemia, with a focus on MLL-AF4 Acute Lymphoblastic Leukaemia (ALL).
I use a wide variety of high-throughput techniques (ChIP-seq, low cell number ChIPMentation, ATAC-seq, RNA-seq and Capture-C ) together with machine learning and other bioinformatic methods to better understand the regulation of chromatin and gene expression.
Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture.
Downes DJ. et al, (2022), Nat Protoc
DOT1L inhibition reveals a distinct subset of enhancers dependent on H3K79 methylation.
Godfrey L. et al, (2019), Nat Commun, 10
Cut-and-Run: A Distinct Mechanism by which V(D)J Recombination Causes Genome Instability.
Kirkham CM. et al, (2019), Mol Cell, 74, 584 - 597.e9
The ESC: The Dangerous By-Product of V(D)J Recombination.
Smith AL. et al, (2019), Front Immunol, 10