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Plasmacytoid dendritic cells (pDCs) produce type I interferon (IFN-I) and are traditionally defined as being BDCA-2+CD123+. pDCs are not readily detectable in healthy human skin, but have been suggested to accumulate in wounds. Here, we describe a CD1a-bearing BDCA-2+CD123int DC subset that rapidly infiltrates human skin wounds and comprises a major DC population. Using single-cell RNA sequencing, we show that these cells are largely activated DCs acquiring features compatible with lymph node homing and antigen presentation, but unexpectedly express both BDCA-2 and CD123, potentially mimicking pDCs. Furthermore, a third BDCA-2-expressing population, Axl+Siglec-6+ DCs (ASDC), was also found to infiltrate human skin during wounding. These data demonstrate early skin infiltration of a previously unrecognized CD123intBDCA-2+CD1a+ DC subset during acute sterile inflammation, and prompt a re-evaluation of previously ascribed pDC involvement in skin disease.

Original publication

DOI

10.1084/jem.20190811

Type

Journal article

Journal

J Exp Med

Publication Date

02/03/2020

Volume

217

Keywords

Antigen Presentation, Antigens, CD1, Dendritic Cells, Humans, Inflammation, Interleukin-3 Receptor alpha Subunit, Lectins, C-Type, Lymph Nodes, Membrane Glycoproteins, Receptors, Immunologic, Skin