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Congenital dyserythropoietic anemia type 1 (CDA-1), a rare inborn anemia characterized by abnormal chromatin ultrastructure in erythroblasts, is caused by abnormalities in codanin-1, a highly conserved protein of unknown function. We have produced 3 monoclonal antibodies to codanin-1 that demonstrate its distribution in both nucleus and cytoplasm by immunofluorescence and allow quantitative measurements of patient and normal material by Western blot. A detailed analysis of chromatin structure in CDA-1 erythroblasts shows no abnormalities in overall histone composition, and the genome-wide epigenetic landscape of several histone modifications is maintained. However, immunofluorescence analysis of intermediate erythroblasts from patients with CDA-1 reveals abnormal accumulation of HP1α in the Golgi apparatus. A link between mutant codanin-1 and the aberrant localization of HP1α is supported by the finding that codanin-1 can be coimmunoprecipitated by anti-HP1α antibodies. Furthermore, we show colocalization of codanin-1 with Sec23B, the protein defective in CDA-2 suggesting that the CDAs might be linked at the molecular level. Mice containing a gene-trapped Cdan1 locus demonstrate its widespread expression during development. Cdan1(gt/gt) homozygotes die in utero before the onset of primitive erythropoiesis, suggesting that Cdan1 has other critical roles during embryogenesis.

Original publication

DOI

10.1182/blood-2010-09-308478

Type

Journal article

Journal

Blood

Publication Date

23/06/2011

Volume

117

Pages

6928 - 6938

Keywords

Anemia, Dyserythropoietic, Congenital, Animals, Carrier Proteins, Cell Line, Tumor, Cells, Cultured, Chromatin, Chromosomal Proteins, Non-Histone, Erythroblasts, Female, Glycoproteins, Humans, Male, Mice, Mice, Inbred C57BL, Mutation, Nuclear Proteins, Vesicular Transport Proteins