Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Resident memory B (BRM) cells develop and persist in the lungs of influenza-infected mice and humans; however, their contribution to recall responses has not been defined. Here, we used two-photon microscopy to visualize BRM cells within the lungs of influenza -virus immune and reinfected mice. Prior to re-exposure, BRM cells were sparsely scattered throughout the tissue, displaying limited motility. Within 24 h of rechallenge, these cells increased their migratory capacity, localized to infected sites, and subsequently differentiated into plasma cells. Alveolar macrophages mediated this process, in part by inducing expression of chemokines CXCL9 and CXCL10 from infiltrating inflammatory cells. This led to the recruitment of chemokine receptor CXCR3-expressing BRM cells to infected regions and increased local antibody concentrations. Our study uncovers spatiotemporal mechanisms that regulate lung BRM cell reactivation and demonstrates their capacity to rapidly deliver antibodies in a highly localized manner to sites of viral replication.

Original publication

DOI

10.1016/j.immuni.2022.03.003

Type

Journal article

Journal

Immunity

Publication Date

12/04/2022

Volume

55

Pages

718 - 733.e8

Keywords

antibody response, humoral immunity, influenza virus, live imaging, memory B cell, mucosal immunity, plasma cells, resident memory B cells, tissue-resident immunity, two-photon microscopy, Animals, Antibodies, Humans, Immunologic Memory, Influenza, Human, Memory B Cells, Mice, Orthomyxoviridae, Orthomyxoviridae Infections